Investigation on the preservation of - acetolactate decarboxylase 酒酒球菌计数方法研究
A - acetolactate decarboxylase is critically important in beer industry . during beer or wine fermentation , the yeast produces a - acetolactate , and the a - acetolactate can be spontaneously decarboxylated into diacetyl 5 )是啤酒工业中使用的一种重要酶制剂,它可以降低啤酒中双乙酰的含量,加快啤酒的成熟,提高啤酒质量。
- acetolactate decarboxylase is purifed from cell extract by 50 % - 80 % ammonium sulfate - fractionation , 50 , 2min heat treatment and deae - sepharose fast flow column chromatography , which we study the different ph and different buffer of deae - sepharose fast flow column chromatography and conclude ph 6 对其酶学性质进行了研究。 -乙酰乳酸脱羧酶经50 80硫酸铵分级沉淀、 50 , 2min热处理、 deae - sepharosefastflow离子交换柱层析方法分离纯化。
- acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms . the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product . in this paper , - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation , heat treatment , deae - sepharose fast flow column chromatography 本文对来源于枯草芽孢杆菌( bacillussubtilis ) 3226 - 5的-乙酰乳酸脱羧酶经硫酸铵分级沉淀、热处理、 deae - sepharosefastflow离子交换柱层析等分离纯化步骤,得到sds - paeg电泳纯,通过n末端氨基酸序列分析验证酶蛋白的纯度。
百科解释
In enzymology, an acetolactate decarboxylase () is an enzyme that catalyzes the chemical reaction